Chromatography Essay examples -- essays research papers

Chromatography is a separation technique in which the mixing to be separated is fade out in a solvent and the resulting solution, often called the mobile phase, is then passed by or over another material, the stationary phase. The separation of the original mixture depends on how powerfully each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not take alone with the mobile phase. Weakly attracted substances will move more chop-chop with the mobile phase.Liquid chromatography is an analytical technique that is useful for separating ions or molecules that are dissolved in a liquid phase. If the sample solution is in contact with a second solid or liquid phase, the divergent solutes will interact with the other phase to differing degrees due to differences in adsorption, garret strength, polarity or size. These differences allow the mixture components to be separated from each other by using thes e differences to determine the transit time of the solutes with a column.Simple liquid chromatography consists of a column with a fritted bottomland that holds a stationary phase in equilibrium with a solvent. characteristic stationary phases (and their interactions with solutes) are solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and porous inert particles (size exclusion). The mixtur...